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국문

구강은 타액으로 인해 항상 침습한 상태를 유지하는 공간으로 세균이 상주하기에 적합한 습도를 가지고 있는 환경이기 때문에 구강의 건강과 병적 상태가 공존하는 관계가 존재하고 있다. 건강한 상태에서 세균총은 안정적인 상태이지만 병적 상태의 세균총은 숙주인 사람의 구강에서 세균막을 형성하여 구취, 치아우식증, 치은염, 치주염을 유발하는 원인이 된다. 상업적으로 이용 가능한 여러 합성 화합물질이 있지만, 이러한 물질은 구강 내 미생물을 변형시킬 수 있기 때문에 천연 약용 식물에 대한 연구가 활발하게 이루어지고 있다. 야관문(Lespedeza cuneata) 추출물의 항균작용으로부터 항산화, 항노화 및 항균성 효과가 있다고 보고되어있지만, 이를 적용하여 구강질환에 대한 항균효과에 관한 연구는 활발한 연구가 이루어 지지 못하여 매우 부족한 실정이다. 따라서, 본 연구는 에탄올에 추출된 야관문(Lespedeza cuneata) 추출물이 구강질환을 일으키는 세균에 대한 항균효과를 통한 항 구강질환의 천연 약제로 사용가능성을 평가하고자 한다. 

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The oral cavity is a space that always maintains an invasive state due to saliva and has a humidity suitable for bacteria to reside in, so there is a relationship between oral health and pathological conditions. In a healthy state, the bacterial gun is in a stable state, but in a pathological state, it forms a bacterial membrane in the mouth of the host person, causing bad breath, dental caries, gingivitis, and periodontitis. Although there are several commercially available synthetic compounds, studies on natural medicinal plants have been actively conducted because these substances may transform microorganisms in the mouth. Although it has been reported that there are antioxidant, anti-aging, and anti-bacterial effects from the antibacterial effects of Lespedezacuneata extract, studies on the antibacterial effects on oral diseases have not been actively conducted, which is very insufficient. Therefore, this study aims to evaluate the availability of Lespedeza cuneata extract extracted from ethanol as a natural drug for oral diseases through antibacterial effects on bacteria that cause oral diseases.

국문

본 연구참여 시작 일주일 전 부산에 위치한 M치과에 내원하여 치과의사의 구강검진을 받고 교육된 치과위생사 2명은 동일한 조건의 구강환경을 위하여 가벼운 스케일링을 수행하였다. 스케일링을 마친 후 회복기간으로 1주일 후 연구가 개시되었다. 치과 방문 시 동일한 칫솔과 치약을 제공하고 칫솔질 후 먼저 baseline으로 치주관련 지표를 확인하기 위한 대표적인 치아로 #16, #21, #24, # 36, #41, #44를 선정하여 측정하였다. 그룹별로 1분 가글 직후의 데이터도 확보하였다. 모든 연구대상자들에게 칫솔질 횟수와 방법, 구강양치액 사용 방법을 교육하였다. 대상자들은 자신이 실험군이지, 대조군인지 알 수 없도록 라벨링하여 제공하였으며, 제공된 치솔, 치약을 사용하여 칫솔질한 다음 잠들기 전 가글을 수행하도록 교육하였다. 5일동안 실험군은 10mg/ml 야관문 추출물15ml 구강양치액을 대조군은 Saline 15ml 구강양치액을 수행하도록 하였다. 가글 후에는 물과 어떠한 음식물 섭취를 허락하지 않았고 이러한 중재 과정은 5일동안 이루어졌다. 임상적 평가로는 올리어리(O’Leary) 지수, 플라크 지수(PI), 치은 지수(GI), 구강 내 미생물 분석 O’Leary index, plaque index (PI), and gingival index (GI), microbiological analysis을 확인하였다. 측정된 데이터 수집은 기준선, 중재 직후, 5일 후 총 3번으로 치과의사지도하에 교육받은 2명의 치과위생사에 의해 확보되었다.

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Two dental hygienists, who visited M dental clinic in Busan 1 week before the commencement of this study and received oral examination and training from a dentist, were given slight oral scaling in order to establish an identical oral environment. The study began 1 week after the scaling due to the recovery period. The toothbrush and toothpaste were provided at the time of dental visit, and maxillary right first molar (#16), maxillary left central incisor (#21), maxillary left first premolar (#24), mandibular left first molar (# 36), mandibular right central incisor (#41), and mandibular right first premolar (#44) were selected as the representative teeth used to identify the baseline periodontal-related index, which was measured before brushing the teeth. Data for each group immediately after gargling for 1 minute were also obtained. All study subjects were educated on the frequency and method of toothbrushing and using mouthwash. The subjects were provided with the labels so that they could not identify whether they are in a study group or a control group. They were also instructed to brush their teeth by using the provided toothbrush and toothpaste and then gargle before going to bed. For 5 days, the study group received 15 mL of 10mg/ml Lespedeza cuneata extract and the control group received 15 mL of saline. After gargling, water or food ingestion was not allowed. This procedure was carried out for 5 days. O'Leary index, plaque index (PI), gingival index (GI), and microbiological analysis were identified with the clinical examination. The measured data were collected for a total of 3 times (Baseline, Treatment, and After 5 Days) and was secured by 2 dental hygienists who were trained under the guidance of a dentist.

상세내용 영문

For 5 days, the study group received 15 mL of 10mg/ml Lespedeza cuneata extract mouthwash. After gargling, water or food ingestion was not allowed. This procedure was carried out for 5 days.

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For 5 days, the control group received 15 mL of saline. After gargling, water or food ingestion was not allowed. This procedure was carried out for 5 days.

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Not Applicable-Etc

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Mmicrobiological analysis

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Baseline before application of gargle solution, treatment immediately after application with gargle solution, 5 days after gargle

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O’Leary index 

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Baseline before application of gargle solution, treatment immediately after application with gargle solution, 5 days after gargle

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Plaque index (PI) 

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Baseline before application of gargle solution, treatment immediately after application with gargle solution, 5 days after gargle

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Evaluation of improvement by test subjects themselves

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Baseline before application of gargle solution, treatment immediately after application with gargle solution, 5 days after gargle

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Gingival index (GI) 

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Baseline before application of gargle solution, treatment immediately after application with gargle solution, 5 days after gargle

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Cariview

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Baseline before application of gargle solution, 5 days after gargle

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Application of mouthwash containing Lespedeza cuneata extract significantly decreased clinical values in all indicators of oral microbes, O’Leary index, PI, and GI (p<0.05). In addition, after gargling with a mouthwash containing Lespedeza cuneata extract, the antibacterial action on Gram-negative and Gram-positive bacteria that cause oral disease showed an excellent clinical effect in inhibiting, preventing, and treating oral diseases. The use of the mouthwash containing Lespedeza cuneata extract was found to be effective for oral desease related indicators and bacteria causing oral disease. Therefore, using a mouthwash containing Lespedeza cuneata extract, a natural drug, will possibly maintain healthy oral health by inhibiting and preventing the progression of oral disease.